1 00:00:06,070 --> 00:00:04,230 the dragon cargo ship which arrived at 2 00:00:08,950 --> 00:00:06,080 the station late last month 3 00:00:12,070 --> 00:00:08,960 carried opals and another large payload 4 00:00:14,070 --> 00:00:12,080 in its unpressurized section its trunk 5 00:00:17,029 --> 00:00:14,080 it also carried 6 00:00:19,189 --> 00:00:17,039 many many other science payloads inside 7 00:00:20,870 --> 00:00:19,199 the freight carrier a number of new 8 00:00:23,670 --> 00:00:20,880 experiments that have come to space 9 00:00:25,509 --> 00:00:23,680 designed to take advantage of the low 10 00:00:27,349 --> 00:00:25,519 earth orbit environment 11 00:00:30,470 --> 00:00:27,359 one of these new experiments is called 12 00:00:33,990 --> 00:00:30,480 biotube micro and it's focused on how 13 00:00:35,830 --> 00:00:34,000 plants sense and respond to gravity the 14 00:00:37,750 --> 00:00:35,840 principal investigator dr carl 15 00:00:39,190 --> 00:00:37,760 hasenstein joins us this morning from 16 00:00:42,310 --> 00:00:39,200 his office at the university of 17 00:00:44,229 --> 00:00:42,320 louisiana at lafayette dr hastenstein 18 00:00:46,310 --> 00:00:44,239 people have been looking at how plants 19 00:00:48,790 --> 00:00:46,320 do in space for a long time 20 00:00:49,750 --> 00:00:48,800 generally how do they do 21 00:00:55,110 --> 00:00:49,760 well 22 00:00:57,590 --> 00:00:55,120 especially once they get exposed to the 23 00:00:58,549 --> 00:00:57,600 proper environment initially we did not 24 00:01:00,389 --> 00:00:58,559 know 25 00:01:02,470 --> 00:01:00,399 what it means to grow 26 00:01:05,750 --> 00:01:02,480 plants in the absence of gravity but it 27 00:01:08,870 --> 00:01:05,760 turns out it wasn't gravity that was the 28 00:01:11,109 --> 00:01:08,880 corporate in not succeeding 29 00:01:13,030 --> 00:01:11,119 with plant growth it was the lack of 30 00:01:15,830 --> 00:01:13,040 convection and as a result the 31 00:01:18,469 --> 00:01:15,840 distribution of gases and so forth that 32 00:01:21,270 --> 00:01:18,479 prevented proper development but we have 33 00:01:23,350 --> 00:01:21,280 come a long way and learned how to do it 34 00:01:25,510 --> 00:01:23,360 in the meantime so we can grow 35 00:01:27,350 --> 00:01:25,520 plants quite well 36 00:01:29,749 --> 00:01:27,360 the gravity is still a factor right what 37 00:01:31,030 --> 00:01:29,759 what caused you to to be focused on that 38 00:01:31,990 --> 00:01:31,040 aspect 39 00:01:34,069 --> 00:01:32,000 well 40 00:01:37,109 --> 00:01:34,079 it's a conundrum for more than a hundred 41 00:01:39,749 --> 00:01:37,119 years to find out why every tree 42 00:01:41,990 --> 00:01:39,759 grows upright and 43 00:01:45,109 --> 00:01:42,000 anchors itself in the soil 44 00:01:49,510 --> 00:01:45,119 and this basic question as to how 45 00:01:51,749 --> 00:01:49,520 tiny cells that don't have specialized 46 00:01:54,870 --> 00:01:51,759 features such as we have in our 47 00:01:58,149 --> 00:01:54,880 vestibular apparatus are able to sense 48 00:02:00,630 --> 00:01:58,159 and respond to gravity is just puzzling 49 00:02:04,069 --> 00:02:00,640 and one of the aspects of 50 00:02:06,389 --> 00:02:04,079 detecting how these things work 51 00:02:09,190 --> 00:02:06,399 ideally requires to 52 00:02:10,630 --> 00:02:09,200 remove gravity as a factor and this way 53 00:02:13,030 --> 00:02:10,640 we can 54 00:02:16,150 --> 00:02:13,040 gradually test how individual components 55 00:02:20,229 --> 00:02:16,160 of this system that makes plants sense 56 00:02:25,670 --> 00:02:22,550 respond to gravity and that's what this 57 00:02:27,589 --> 00:02:25,680 experiment is about okay uh take us 58 00:02:29,110 --> 00:02:27,599 through your plan then what are you 59 00:02:30,309 --> 00:02:29,120 going to be what have you sent to space 60 00:02:33,030 --> 00:02:30,319 and what are you going to be doing in 61 00:02:34,150 --> 00:02:33,040 that hardware on orbit 62 00:02:37,990 --> 00:02:34,160 well 63 00:02:39,830 --> 00:02:38,000 the biotube micro is an external 64 00:02:41,190 --> 00:02:39,840 shell that contains the actual 65 00:02:42,229 --> 00:02:41,200 experiment 66 00:02:44,390 --> 00:02:42,239 and 67 00:02:45,830 --> 00:02:44,400 the actual experiment consists of 68 00:02:47,750 --> 00:02:45,840 so-called 69 00:02:49,190 --> 00:02:47,760 magnetic 70 00:02:54,630 --> 00:02:49,200 chambers 71 00:02:57,270 --> 00:02:54,640 series of exactly 10 72 00:02:59,430 --> 00:02:57,280 magnets that are aligned so that they 73 00:03:01,430 --> 00:02:59,440 generate a very strong 74 00:03:04,550 --> 00:03:01,440 magnetic field 75 00:03:07,830 --> 00:03:04,560 inside this field we distort this 76 00:03:09,509 --> 00:03:07,840 uniform magnetic field by 77 00:03:13,509 --> 00:03:09,519 you could call it 78 00:03:15,910 --> 00:03:13,519 magnetic lenses there in reality 79 00:03:17,830 --> 00:03:15,920 ferromagnetic wedges 80 00:03:20,790 --> 00:03:17,840 and what does that have to do with how 81 00:03:23,589 --> 00:03:20,800 plants respond to gravity these magnetic 82 00:03:27,430 --> 00:03:23,599 gradients that result by inserting these 83 00:03:30,550 --> 00:03:27,440 wedges into the uniform magnetic field 84 00:03:31,750 --> 00:03:30,560 exert what we call a magnetophoretic 85 00:03:34,869 --> 00:03:31,760 force 86 00:03:38,630 --> 00:03:34,879 is specific 87 00:03:41,110 --> 00:03:38,640 for diamagnetic compounds such as the 88 00:03:43,589 --> 00:03:41,120 middle class that are the suspected 89 00:03:46,470 --> 00:03:43,599 gravity sensors it sounds like you're 90 00:03:49,190 --> 00:03:46,480 you're manipulating the gravity pull 91 00:03:51,990 --> 00:03:49,200 on on on these parts of the plant 92 00:03:54,710 --> 00:03:52,000 that's correct but we don't exert a pull 93 00:03:56,869 --> 00:03:54,720 we exert a push 94 00:03:58,789 --> 00:03:56,879 these high graded magnetic fields repel 95 00:04:00,630 --> 00:03:58,799 the starch 96 00:04:02,789 --> 00:04:00,640 filled the miloplasts 97 00:04:03,670 --> 00:04:02,799 and by doing so 98 00:04:06,149 --> 00:04:03,680 they 99 00:04:08,470 --> 00:04:06,159 produce the same effect that we see 100 00:04:11,350 --> 00:04:08,480 under the influence of gravity 101 00:04:14,550 --> 00:04:11,360 without having any other 102 00:04:16,629 --> 00:04:14,560 cellular component exposed to gravity so 103 00:04:17,909 --> 00:04:16,639 we can pinpoint whether the milloplast 104 00:04:19,349 --> 00:04:17,919 is actually 105 00:04:21,430 --> 00:04:19,359 doing 106 00:04:23,350 --> 00:04:21,440 the the initiation 107 00:04:27,510 --> 00:04:23,360 of the sensing and 108 00:04:31,189 --> 00:04:27,520 responds to gravity by virtue of 109 00:04:33,590 --> 00:04:31,199 moving them around in their respective 110 00:04:35,909 --> 00:04:33,600 cells we call them static sites very 111 00:04:38,790 --> 00:04:35,919 interesting do the do the crew members 112 00:04:41,350 --> 00:04:38,800 participate in this in some way 113 00:04:42,629 --> 00:04:41,360 not really they were 114 00:04:44,710 --> 00:04:42,639 helpful in 115 00:04:47,990 --> 00:04:44,720 stowing the the hardware into the 116 00:04:49,909 --> 00:04:48,000 express rack on iss but the actual 117 00:04:52,790 --> 00:04:49,919 experiment is 118 00:04:55,749 --> 00:04:52,800 entirely controlled from ksc 119 00:04:57,030 --> 00:04:55,759 okay now i assume then that 120 00:04:59,030 --> 00:04:57,040 you've flown this on dragon you're going 121 00:05:00,469 --> 00:04:59,040 to get these samples back what do you do 122 00:05:02,390 --> 00:05:00,479 with them when you get them 123 00:05:03,590 --> 00:05:02,400 well we we will 124 00:05:04,870 --> 00:05:03,600 analyze 125 00:05:06,469 --> 00:05:04,880 different things 126 00:05:08,790 --> 00:05:06,479 one is the distribution of these 127 00:05:11,670 --> 00:05:08,800 amyloplasts 128 00:05:13,590 --> 00:05:11,680 after we have seen downlink video to 129 00:05:16,390 --> 00:05:13,600 indicate whether we obser observe 130 00:05:18,469 --> 00:05:16,400 actually curvature in the roots so we 131 00:05:20,150 --> 00:05:18,479 have a video equipment 132 00:05:22,469 --> 00:05:20,160 that takes 133 00:05:23,749 --> 00:05:22,479 pictures in the infrared region of this 134 00:05:30,469 --> 00:05:23,759 um 135 00:05:33,670 --> 00:05:30,479 come back 136 00:05:35,430 --> 00:05:33,680 we will call we will correlate the 137 00:05:37,430 --> 00:05:35,440 distribution of 138 00:05:40,390 --> 00:05:37,440 the middle class within these status 139 00:05:42,310 --> 00:05:40,400 sides to the observed curvature and we 140 00:05:45,590 --> 00:05:42,320 will also 141 00:05:48,469 --> 00:05:45,600 do a series of genetic tests to see if 142 00:05:49,670 --> 00:05:48,479 gene expression has changed 143 00:05:52,629 --> 00:05:49,680 in the 144 00:05:56,029 --> 00:05:52,639 growth conditions that we had in space 145 00:05:58,150 --> 00:05:56,039 at the same time we do so called 146 00:06:00,309 --> 00:05:58,160 immunocytolocalization of relevant 147 00:06:01,909 --> 00:06:00,319 proteins that are responsible for the 148 00:06:03,909 --> 00:06:01,919 growth response of 149 00:06:05,749 --> 00:06:03,919 cells and or roots in general so there's 150 00:06:07,350 --> 00:06:05,759 still plenty of study to be done once 151 00:06:09,510 --> 00:06:07,360 you get the samples back 152 00:06:11,830 --> 00:06:09,520 yes indeed now i found it very 153 00:06:13,990 --> 00:06:11,840 interesting that i saw that you ran this 154 00:06:15,830 --> 00:06:14,000 experience experiment on the last flight 155 00:06:17,830 --> 00:06:15,840 of columbia and we're still able to get 156 00:06:20,790 --> 00:06:17,840 some results back from downlinked images 157 00:06:22,950 --> 00:06:20,800 right yeah that's correct um it it was 158 00:06:25,270 --> 00:06:22,960 unfortunate that we could not get our 159 00:06:28,469 --> 00:06:25,280 biological material back 160 00:06:31,749 --> 00:06:28,479 but the downlink suggested strongly that 161 00:06:33,590 --> 00:06:31,759 we had curvature as we had expected but 162 00:06:36,230 --> 00:06:33,600 we were not able to confirm this because 163 00:06:39,510 --> 00:06:36,240 we never received the actual 164 00:06:41,430 --> 00:06:39,520 plant material is your goal to simply 165 00:06:43,830 --> 00:06:41,440 improve the way plants grow in space or 166 00:06:44,710 --> 00:06:43,840 does this have applicability on earth 167 00:06:46,870 --> 00:06:44,720 well 168 00:06:49,909 --> 00:06:46,880 any time you have 169 00:06:51,029 --> 00:06:49,919 better or deeper understanding as to how 170 00:06:53,189 --> 00:06:51,039 plants 171 00:06:55,830 --> 00:06:53,199 perform and respond to 172 00:06:57,510 --> 00:06:55,840 environmental cues or problems 173 00:07:00,309 --> 00:06:57,520 you are bound to 174 00:07:01,270 --> 00:07:00,319 benefit processes on earth as well 175 00:07:04,309 --> 00:07:01,280 so 176 00:07:07,110 --> 00:07:04,319 while we clearly want to 177 00:07:09,909 --> 00:07:07,120 establish a means of directionally 178 00:07:11,589 --> 00:07:09,919 controlling plant growth in space we 179 00:07:15,270 --> 00:07:11,599 also 180 00:07:18,629 --> 00:07:15,280 want to use that gained knowledge for 181 00:07:20,870 --> 00:07:18,639 improvement of plants on on earth 182 00:07:22,309 --> 00:07:20,880 dr hazenstein thank you for taking a few 183 00:07:24,150 --> 00:07:22,319 minutes for us this morning and good 184 00:07:26,390 --> 00:07:24,160 luck with the experiment thank you so